Alkaline Phosphatase anticorps

N° du produit ABIN94942

N° du produit (Fournisseur): 100-4134

Cet anticorps Lapin Polyclonal détecte spécifiquement Alkaline Phosphatase dans WB et ELISA. Il présente une réactivité envers E. coli et a été mentionné dans 2+ publications.

Aperçu rapide pour Alkaline Phosphatase anticorps (ABIN94942)

Antigène: Alkaline Phosphatase (ALP)

Reactivité: E. coli

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Alkaline Phosphatase est non-conjugé

Application: Western Blotting (WB), ELISA

Détail du produit anti-Alkaline Phosphatase anticorps

N° du produit (Fournisseur): 100-4134

Fournisseur: Rockland

Fonction: Alkaline Phosphatase Antibody

Attributs du produit: Synonyms: rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody

Purification: Anti-Alkaline Phosphatase (E.Coli) Antibody was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogène:

Immunogen: Alkaline Phosphatase [E.coli]

Immunogen Type: Native Protein

Information d'application

Indications d'application:

Application Note: Anti-Alkaline Phosphatase (E. Coli) Antibody has been tested against 1.0 μg of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for Anti-Alkaline Phosphatase (E. Coli) Antibody.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:8,000 - 1:40,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 75 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Références pour anticorps anti-Alkaline Phosphatase (ABIN94942)

Epstein, Kiechle, Artiss, Zak: "The clinical use of alkaline phosphatase enzymes." dans: Clinics in laboratory medicine, Vol. 6, Issue 3, pp. 491-505, (1986) (PubMed).

Lange, Millan, Stigbrand, Vessella, Ruoslahti, Fishman: "Placental alkaline phosphatase as a tumor marker for seminoma." dans: Cancer research, Vol. 42, Issue 8, pp. 3244-7, (1982) (PubMed).

Détails sur Alkaline Phosphatase

Antigène: Alkaline Phosphatase (ALP)

Autre désignation: Alkaline Phosphatase

Sujet: Background: Alkaline Phosphatase is an enzyme that is key in the hydrolysis of many different types of molecules, resulting in the removal of phosphate groups from said molecule. Most active in basic environments and a common serum analyte, alkaline phosphatase and its isoenzymes are good indicators of bone, liver, and testicular diseases/cancers in immunoassays and diagnostics. Anti-Alkaline Phosphatase (E. Coli) Antibody is ideal for investigators in Cancer, Immunology, and Molecular Biology.

ID gène: 945041

NCBI Accession: NP_414917

UniProt: P00634